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Anti -IDH1 Rabbit pAb

货号:GB111194

品牌:Servicebio

价格:¥650

规格: 100μl

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Product Information

 

Description

IDH1 rabbit polyclonal

Protein full name
Isocitrate dehydrogenase [NADP] cytoplasmic

Synonyms

Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH, Idh1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse IDH1

Isotype IgG
Purity Affinity purification

Predicted MW.

47 kDa

Observed MW.

47 kDa

Uniprot ID

O75874, O88844, P41562

 


 

Applications

 

Applications

WB

Species

Human, Mouse, Rat

Dilution

1: 500-1: 1000

Positive tissue

liver, kidney, stomach

 


 

Background

 

The Isocitrate dehydrogenase cytoplasmic enzyme belongs to the isocitrate and isopropylmalate dehydrogenases family. IDHC catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of ?ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Ca2+ can bind to IDHC as a complex with isocitrate, acting as a competitive inhibitor of Mg2+. The IDHC enzyme is inactivated by phosphorylation at Ser-113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock.
 


 

Images

 

Western blot analysis of IDH1 (GB111194) at dilution of 1: 1000
Lane 1: C2C12 cell lysate
Lane 2: Raji cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Mouse stomach tissue lysate
Lane 7: Rat liver tissue lysate
Lane 8: Rat kidney tissue lysate
Lane 9: Rat stomach tissue lysate
 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

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